The Catalytic Subunit B-family of Replicative DNA...

The catalytic subunit-PolÃŽ ± Polymerase ÃŽ ± is the first mammalian polymerase detected. It is a member of the B-family of replicative DNA polymerases. Its primary role is to further extend the RNA primer by addition of deoxy-nucleotides (dNTPs) into a total of 20-25 nucleotides of RNA-DNA primer. Generally, replicative DNA polymerases, polymerize dNTPs by catalyzing nucleophilic attack of the 3’-hydroxyl group of the primer onto the ÃŽ ±-phosphate of an incoming dNTP aligned to the template strand. The accuracy of the polymerases is achieved by a confirmation of the correct base pairing of the incoming dNTP and the template strand according to Watson-Crick base-pairing. The accuracy of the primases is further enhanced (100 fold) by their exonuclease activity where in the event of a mismatch the growing strand is pushed back to the exonuclease active and the mismatched nucleotide is excised. Most of our structural knowledge of the B-family of DNA polymerases came from the structure of the prototypical family member, bacteriophage RB69 Pol (Wang et al., 1997 in elife). Followed that the structure of the bacterial DNA Pol II (Wang and Yang, 2009 in elife) and then yeast Pol ÃŽ ´ (Swan et al., 2009). Only recently, the crystal structure of the catalytic domain of the yeast Pol ÃŽ ± subunit, Pol ÃŽ ± 349–1258, has been solved (elife). The structure confirmed that Pol ÃŽ ± shares a polymerase and an exonuclease domains similar to RB69 Pol, bacterial DNA Pol II and yeast Pol ÃŽ ´. Unlike the otherShow MoreRelatedDna Analysis : Dna And Dna2142 Words   |  9 Pages DNA polymerases are vital in how an organism can sustain life. DNA polymerases are enzymes that synthesize DNA molecules from deoxyribonucleotides and are accountable for DNA replication. They are absolutely critical for DNA replication and will typically work in sets so that they can create two identical sets of DNA strands from one single strand of DNA. DNA polymerase will catalyze the reaction: deoxynucleoside triphosphate + DNAn diphosphate + DNAn+1. DNA polymerases are extremely important

The Salem Witch Trials Of 1692-93 - 2316 Words

When examining the topic of the Salem witch trials of 1692-93, Cotton Mather is presumed to be a major cause behind the hysteria of the events. He contributed to this image, perhaps unknowingly, by publishing numerous works especially â€Å"Wonders of the Invisible World.† At first glance, Mather s book seems to be just a detailed account of the trials and executions that took place in the Massachusetts colony, but deeper investigation reveals otherwise. To dispel Mather s arguments and reveal more details on the events of Salem at this time, Robert Calef published â€Å"More Wonders of the Invisible World† in 1700. Calef s book seems just as capable as Mather s book to maintain longevity in the world of literature and American history, however Mather has held a much more prominent stance out of the two. By examining Cotton Mather s obligation to God and comparing the language of Mather and Calef, we develop more understanding as to why his arguments remain more promine nt than Calef s as well as how both of the authors influenced literature and American history in general. Cotton Mather s strenuous attempts to live up to the image of his father, Increase Mather, is one factor contributing to his form of writing. He was also influenced especially by his Calvinist upbringing that taught him to take life seriously and resort to prayer in time of need. This deeply religious upbringing helped Mather to develop a sense of self-discipline at a young age. He was proficient inShow MoreRelatedAbdeali Dalal. Dr. Jenkins. History 1301. February 13,1541 Words   |  7 Pagesâ€Å"Escaping Salem; The Other Witch Hunt of 1692† â€Å"Escaping Salem; The Other Witch hunt of 1692† was penned and published by Richard Godbeer in 2005. Godbeer expressed his views on what he called basic witch trials in Salem and more importantly, in Stamford, Connecticut Massachusetts. The same year that Salem witch hunt and trials began in 1692, another witch hunt took place in Stamford, the Western Connecticut to when a woman (Elizabeth Clawson) from there was also accused of Witchcraft. These witch huntRead MoreThe Events And History Of Salem Witch Trials799 Words   |  4 PagesThe Events and History of the Salem Witch Trials Katie Duggan†¨World history period 4†¨Mr. Whittemore†¨26 February 2016 We have been blaming others for our misfortunes since before the Roman Empire. The Salem witch trials started through a little girl s illness and spread to other men, women and children. The events that took place during the Salem Witch Trials in 1692 is part of a pattern through our history to persecute innocent people. Belief in the devil’s practice of giving certainRead MoreThe Crucible By Arthur Miller1441 Words   |  6 PagesCrucible was based off the events in a small town/ village called Salem in 1692. There were no more than 600 residents all together so everyone knew each other. The Crucible focused on the Salem witch trials that took place, and started from young girls claiming to be possessed by the devil. The people who lived in Salem were so obsessive about the possible threats in their town that they came up with unreasonable reasons on who was a witch, which made many innocent people die. Many innocent people wereRead MoreThe Salem Witch Trials : Questionable And Scandalous Time1010 Words   |  5 PagesThe Salem Witch Trials were a questionable and scandalous time in New England history that led to the deaths of 14 women and 6 men, all of whom had been charged with practicing witchcraft. The trials were the result of the strange and unexplainable behavior of two young girls , who exhibited violent convulsions and uncontrollable outburst of screaming and fits that left them unable to see,speak or hear. After a local doctor examined them and found no physical reason for their fits, diagnosed themRead MoreThe Salem Witch Trials : A Day By Day Chronicle Of A Community Under Siege Essay1671 Words   |  7 PagesThe book I have chosen to write about is â€Å"The Salem Witch Trials: A Day by Day Chronicle of a Community Under Siege† written by Marilynne K. Roach and published in 2002. Marilynne K. Roach is the president of the Historical Society of Watertown and a member of the Watertown Historical Commission board, she also is an active board member and a curator of the Salem Witch Museum. She has multiple books that have been published about the Salem and the trials that occurred in the late 17th and early 18thRead MoreSalem Witch Hunt : A Phenomenon Of Witch Trials1499 Words   |  6 Pages The Salem Witch Hunt was a phenomenon of witch trials in the Early Modern period. It occurred in 1692 in Salem, Massachusetts. It all started when a group of young girls, Betty Parris, 9 years old, and Abigail Williams, 11 years old, started to behave in a crazy manner, like screaming and barking like a dog, and it got to the point where they assumed they were â€Å"possessed†. Soon after that, Ann Putnam Jr. and other Salem girls began acting similarly. People started getting very scared and startedRead MoreThe Salem Of Witch Trails2281 Words   |  10 PagesBlumberg, the Salem of witch trails was a really bad part of the United State. There were people who didn’t understanding why they were getting accused. This happed during 1692 and 1693 in Massachusetts. This happened during this time. The main parts that started and fueled the trials were politics, religion, family, feuds, economics, and the imaginations and fears of people (Sutter). The seeds of the hysteria is afflicted Salem village, Massachusetts were sown on January 1692 when groups ofRead MoreThe Crucible By Arthur Miller Essay1646 Words   |  7 Pagesin the play. Although Arthur Miller’s The Crucible is set in the late 1600s, it offers a fine example of allegory of McCarthyism in the 1950s. During the late 1600s in Salem, Massachusetts, people are experiencing a somewhat mass hysteria as the witch trials occur. The main plot of the play revolves around a group of people in Salem. In begins with Abigail Williams and other girls being questioned about practicing witchcraft when a young Betty Parris is found motionless and unconscious. While beingRead MoreCommunism And Communism In Arthur Millers The Crucible711 Words   |  3 Pagesanalogy using the events of the Salem Witch Trials of 1693 to expose the ugly truth behind communism and McCarthyism. To begin, the word crucible derived from the Latin root word â€Å"crux† means cross. In explanation, a crucible is a severe test or trial, which is precisely what occurs in the play. Ordinarily, â€Å"The Crucible† inked in 1952, is a dramatization of The Salem Witch Trials that took place in Salem, Massachusetts during 1692-93. The Crucible examines witch hunts that took place withinRead MoreThe Salem Of Witch Trails2303 Words   |  10 PagesBlumberg, the Salem of witch trails it was a really bad part of the United State. There were people who didn’t understanding why they were getting accused. This happed during the 1692 and 1693 in Massachusetts this was really happing during this time. The main parts that started and fueled the trials were politics, religion, family, feuds, economics, and the imaginations and fears of people (Sutter). The seeds of the hysteria is afflicted Salem village, Massachusetts were sown on January 1692 when groups

Cuisine And Culture Of The Restaurant †MyAssignmenthelp.com

Question: Discuss about the Cuisine And Culture Of The Restaurant. Answer: Cuisine And Culture Of The Restaurant: I went to dine in the Host India restaurant situated in Ottawa, Canada. The Host India restaurant is known for fulfilling the wish of those food consumers in Canada, who crave for Indian foods. It mainly provides all dishes that is popular in Indian cuisine and provides authentic Indian food both in the form of buffet as well as delivery/take out. All contemporary Indian meals and desserts are available there (Hostindiaottawa.ca. 2018). Review Of Dining Experience At The Restaurant: I and my friend went to the Host India restaurant to try a different cuisine, however we had little idea about Indian cuisines. However, the experience there was exceptional as the manner in which they presented their foods to us, I got to know a lot about Indian cuisines. The first thing that attracted out attention while entering the restaurants was interior and exterior dcor of the restaurant. Unlike other classic restaurant in Canada, it had Indian decorations instead of Italian decorations. The ambience may not be termed as exceptional or unique, it had basic dining tables and buffet areas arranged for guest. However, everything looked sleek and clean. This impressed me because hygiene is a priority for me when I visit a restaurant. Based on the dcor and cuisine, it can be said that the restaurant acted an ethnic restaurant that used their dining services to attract the attention of tourist. Through their focus on maintaining authenticity of Indian food both in dcor as well as f ood served, they engaged in good expression of identity. The notion of authenticity as a motivation for promoting culinary tourism was maintained (Molz 1998). As I had no idea about the cuisine, I was a little clueless about the menu presented to us. This is because we were mainly used to Italian and European cuisines and we were confused regarding the food we should order. Once the staff realized that we were not acquainted with Indian cuisine, he approached us in a delightful manner to explain about the specialty of Indian cuisines and the food that is a must to try here. They were interest not only in serving food but also in striking a cord with us. They asked us questions like Have you dined here before? and what type of food you wish to eat here?. This type of communication strategy helped us to understand the type of flavor and spices we can expect here. Hence, we finally ended up ordering the classic samosa for starters and butter chicken with naan after we got to know that these are the popular items in Indian cuisine. This experience also taught us how food can be a good vehicle for tourism. They were involved in excellent culina ry tourism by their communication strategies. Restaurant staffs acts as the active agent, who help people who are not familiar with a cuisine to construct meaning of the food and fetish the exotic food options. The strategy used by restaurants to construct and negotiate identity about an unknown cuisine is understood (Beagan et al. 2015). In addition, while we were served the food, the staffs at Host India maintained the edibility and palatability value of the food by their presentation styles. Each food items that we ordered were nicely presented and one staff was giving us good information about the spices in each food items. The cutleries and other items at the table were also arranged according to Indian dining styles. They never left us unattended and came back during the meal to ask if we were enjoying the dish. Their attentive service was a winning point for me as their attitude showed that they were willing to provide good dining experience to us. This is also understood from the concept of culinary tourism perspective as Indian food is a rare food item in Canada and by using their food presentation technique and interest in making us familiar with the cuisine, they were promoting interest of diners in Indian cuisine. This strategy to introduce guest to new food by means of good dining experience and providing information about ingredients promotes culinary tourism and ensures that people who are not familiar with cuisine eats the food with pleasure instead of curiosity (Long, 1998).. As a person who loves to experiment with different food items, the experience of visiting the Host India restaurant provided me the opportunity to engage in connoiseeur cosmopolitanism. Connoiseeur cosmopolitanism focus on learning about different international foods and cuisines and the staffs at Host India helped me a lot in giving me knowledge about Indian cuisines (Beagan et al. 2015). They engaged in constructive tourism too as after the meal they asked us whether anything should be changed about dining experience there. I liked the service, the presentation style, however I wanted a better ambience. Due to my curiosity for Indian foods, I visited the website and Facebook page of the restaurant too after the dining experience. The manner in which they have introduced them in the website is very appealing for me. This is because it mentioned in great detail mainly about the Indian meals and the details about the cuisine motivated me to visit again (Hostindiaottawa.ca. 2018). References: Beagan, B.L., Chapman, G.E., Johnston, J., McPhail, D., Power, E.M. and Vallianatos, H., 2015.Acquired tastes: Why families eat the way they do. UBC Press. Hostindiaottawa.ca. 2018. Host India Food Delivery Takeout Menu | Ottawa. Retrieved 5 March 2018, from https://www.hostindiaottawa.ca/?utm_source=googleutm_medium=organicutm_campaign=website Long, L. M. (1998). Culinary tourism: A folkloristic perspective on eating and otherness.Southern Folklore,55(3), 181. Molz, J.G., 1998. Tasting an imagined Thailand.Culinary Tourism,55(3), p.53.

Once Upon a Time... free essay sample

Kelly McDermott WRTG 1 1 50/Albert Literacy Narrative Final 28 August 2010 Once upon a time Writing does not happen like it does in fiction, with inspirational background music, and a sudden appearance ofa beautiful Greek muse. Writing is easy. You only need to stare at a blank piece of paper until drops of blood form on your forehead. (Gene Fowler). People do not sit for hours in front of a computer screen, fghting with a word-processers grammar, because it is fun. Writing can be either worse than the fires from Dantes famous inferno or more lovely than true love, but, n either case, often a needed explosion of brain cells. For me, forming meaning from what started out as inscriptions on cave walls is more than creating art or a little need, but essential. It is not only what I do. Writing is my identity. Delving into my fresh idea for my novel this summer, I found a book that told me how to write a novel in a month. We will write a custom essay sample on Once Upon a Time or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Even though I did not in fact write half a novel in a month, several things the author said still are a large part of my everyday writing experience. An exercise he suggested the reader do was to sit down with some background music (l chose Sun Dance: Summer Solstice), and write for fifteen minutes about something always wanted/wished for in our own life. What I did write is surprisingly personal to me, but I will say that even now reading it will still shock me; I had no clue that I had the feelings that I did bottled up inside. I remember the only conscious thought I had during the entire writing burst was hmm I guess I kinda would like Then I finished writing and my soul was peeking at me between the scrawls on the scrap paper I had decided to use for this useless looking exercise. Eighth grade was the ear that I decided: I would write a novel, I would have it published, and I would be famous. I still have the handwritten original twenty or so pages I wrote five years ago. However, my novel has taken on a completely new basic plot as a freshman in college. I had teachers who taught me how to write using the amazingly in-depth grammar available to me, and I had teachers who taught me about life itself, and that is what my novel is now about: life (using parapsychology to give it a supernatural mood). My favorite teacher in up through high school my best friends mom, referred o as either Mom or Mrs. Thompson. She was my tenth grade honors comp-lit teacher, my humanities one and two teacher, and she was my advanced placement literature instructor senior year. She scared me. Still does usually. Her grading was not what terrified my senior class, or her Look (although that was rather effective when done at the right moment), but rather, her disappointment. When I wrote something horrible in Mrs. Thompsons class I did not care what the number written on the bottom was All I cared about was actually whether she liked it or not, and hether I had her time had been wasted in the end or not. The books we read in my literature class were not assignments; they were gifts from Mrs. Thompson. Read well-written novels that spoke morals? YES! Reading for classes and for fun in free people that I was reading the Harry Potter books by J. K. Rowling when I was in second grade and reading adult level books in middle school. Personally, I am surprised that I did not decide earlier in my life than the last year in my middle school that writing a novel would be a good idea. What actually surprises me most is what has helped me ost with my actual writing: a website. Goodreads. com has a spot where one can search for quotes from authors and famous people and I have always enjoyed reading quotes. When I began reading quotes from Stephan King, however, I began to improve all my writing, not Just my little novel. Not that I have actually read any of his stories myself. Only his quotes and his book On Writing, which discusses his own writing advice and experience. It has been enough to make him one of my favorite author role models. He makes me laugh, feel inspired, and most importantly, he akes me get off my writers block by telling me simply, you can, you should, and if youre brave enough to start, you will. Too many books on writing tell the aspiring writers who read them to let inspiration come to them, but that is nothing like any writing realistically works. miou [cannot] wait for inspiration. You have to go after it with a club Cack London). Writing a paper that delves into the rhetoric of Heart of Darkness by Conrad will not be affected much by inspiration, and writing a poem on the beauty of a tree should bypass conscious inspiration if done correctly. Writing, to me, is saying what I cannot always handle thinking, or rather, my thoughts draining from my brain onto a page without passing my conscious go. For years, Journaling was to me as numbers are to an accountant: no longer exciting, but it was what I did. Writing in a Journal became almost an obligation to tell the invisible person inside the pages the important things happening in my life. Suddenly, in high school, writing developed a completely different meaning in my world, the change beginning with the mystical quality of poetry. The first poem I wrote for my ninth grade honors comp-lit class assignment shocked me with its intensity. We were each given a list of twenty words, and were to choose ten to have in our individual poems, and focus a theme on. It was not the last poem I wrote. Now when I write a poem, Journal, or work on my novel, doing so is not an obligation, doing so could often be considered enlightening by the insight I will often get inside of my own mind. When I picture a poet, I either see my friend Juliana leaning over a tiny notebook in class or Emily Dickenson, sitting by a window, closed off from the world. A novelist? A gray bearded man typing away at an old-fashioned typewriter. Yet here I am, lying on my carpet in my dorm room with a laptop, listening to show tunes and Pandora Radio, and I consider myself in the same league. My goal? Writing isnt about making money, getting famous , or making friends. In the end, it is about enriching the lives of those who will read your work, and enriching your own life, as well. It is about getting up, getting well, and getting over. Getting happy, okay? Getting happy (Stephan King).

GLUEP BALL EXPERIMENT Essays - Matter, Manufacturing,

GLUEP BALL EXPERIMENT Darina Vrublevskaya Observations TRIAL ONE TRIAL TWO BALL REST Does not maintain its shape Maintains its shape BALL BOUNCE Does not bounce and sticks onto the desk Does bounce, about 15 cm up THIN STRIP The strip pulls off and falls The strip holds together PULL ENDS SLOWLY Falls apart Gluep stretches far PULL ENDS QUICKLY Falls apart Snaps into two Questions In glue t he tangled molecules make glue thick and viscous rather than thin and runny. When glue is exposed to air, the water evaporates, leaving the tangled polymer molecules. The tangled molecules stick to the surfaces on which they dried, and hold the surfaces together. Borax has ions can form links between the long, thin polymer molecules in the glue which makes Gluep more like a solid than the plain liquid glue. It allows holding its shape for a short time, and as long as it is not strained. When Gluep rests, the flexible network gradually relaxes, and the Gluep flattens. When Gluep is stretched quickly, the links between molecules break, and the Gluep snaps apart into pieces. Gluep contains a lot of water that contributes to the liquid-like properties to Gluep . If the Gluep is left exposed to open air, the water will evaporate, and the Gluep will gradually stiffen. Altering the materials affected the consistency of the Gluep ball because each substance that was added into the mixture had different types of ions which made the texture different, and the Gluep ball act differently too. This is all linked to stoichiometry because it is the relationship between the relative quantities of substances taking part in a reaction or forming a compound, typically a ratio of whole integers . In this experiment, we compared substances and have observed different types of reactions to them. Works Cited FLINN Scientific Canada. (2013). Glue Slime. Retrieved from https://www.flinnsci.ca/glue-slime/dc91643/ SciFun . (2014). Gluep - Solid or Liquid? Retrieved from http://scifun.che m. wisc.edu/HomeExpts/gluep.htm

Long Term Career Goals For Students in the School of History

Long Term Career Goals For Students in the School of HistoryMany students have the opposite problem, and they don't have a good idea about writing a sample essay for a PhD in History. Some of these students are really not sure what they should write about. This is understandable, because many of them assume that the goal is to achieve a PhD in History or some other university curriculum.In fact, it is so much more than that. The Ph.D. in History requires a completely different type of essay. Therefore, it is imperative to understand the many requirements that make up a sample for this specific objective.A Ph.D. student should have strong teaching qualities. In fact, there is a very specific theme that is required. This can be found on the Department of History website, as it is entitled 'PhD Essay Format and Theme.'The thesis statement is a five-sentence statement that summarizes the main focus of the thesis paper. It has the same basic format as the professor's outline. The authors should start by writing one paragraph. That is followed by four paragraphs that discuss a particular theme.The last paragraph has to be the conclusion and discuss a research or event from the thesis. The author should also include sources where he/she has consulted for information. After writing all of the paragraphs, there is a list of questions that is called an appendix.An organization will want a few sentences that describe their specific topic and structure. The academic year for this specific topic is included in the sample.For the doctoral candidate who is not yet a tenure-trackfaculty member at a major university, it is absolutely imperative to write a good presentation. The presentation should be well organized and clearly written. It should address what it is that students should learn, the standard and is there something else that they should consider as well.As a student who has started thinking about graduate school, it is very important to recognize that there are many short term career goals for students to pursue. However, this does not mean that the longer term goal is unimportant. In fact, students should always look for options that offer options that are a bit outside of their current realm of specialization.

The Vist

The Vist The night sky of Neton was a clear one. The deep blue,almost a black but well illuminated by the bright fullmoon, glistened with a pattern of twinkling stars. Thebackground light from Brisbane was almost nonexistent,a pleasant change of atmosphere that was rarely appreciatedby most.Except Jane. She loved the clear sky, the faint presence ofthe Milky Way as it wrapped itself across the near-endlessplane that was the sky She stood out on the balcony, staringinto the azure. Was there life out there? she wondered. Theuniverse is so huge, why not? Besides, with all the strangethings that happen in the world you'd think that there mightas well be aliens to make the group whole.A streak of light suddenly pierced the night's sky and left adazzling trail of cosmic dust behind it. Jane gasped in awe.A falling star.360Â ° panorama of Racetrack Playa in Death Valley ...The brightest she had seen! She quickly closedher eyes and made a wish, knowing that a wish from a falling starhad excellent ch ances of coming to pass. But the wish had to bestrongly formed in one's mind, or else it would dissipate with allthe other bits of wishful thinking that took place in the night.She tried to form an image, . She almost had it. She slowlyopened her eyes and visualized... Max. Standing at thedoor and was looking at her with an air of mild curiosity.'HEY Jane' he yelled! ' What the hell are you doing ?'Jane nearly fell backwards. He was no vision!That idiot was once again eavesdropping on her!'I WAS making a wish, you jerk!' she blurted out, 'You'vejust ruined it, though!''Making wishes ? Don't bother. No amount of wishingwill make you a better...